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Số 07 (2017) Trang: 58-64
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Article info.



Received 05 Oct 2016
Revised 14 Mar 2017

Accepted 31 Oct 2017


Bacteria naturally occurring in Artemia culture systems act as direct food for the culture organism and also food digestion promoter. However, in the open culture conditions, that, Artemia consumes plenty of food sources available in the pond, makes it difficult to evaluate the role of associated bacteria in growth enhancement. Therefore, this study was conducted under gnotobiotic culture conditions to evaluate the effect of associated bacteria on growth and survival of Artemia. Bacteria-free baker´s yeast Saccharomyses cerevisiae strain wild-type (WT) was used as a main food source for bacteria-free Artemia nauplii in 6 days of culture. Nine unidentified bacteria strains (HT1-HT9) were isolated from culture water of good performance Artemia in our preliminary test, some of these isolates had proven as a diet for Artemia, bacteria were added to Artemia culture only one time at a pre-tested concentration of 5x106 cells/mL. The results of this study showed that Artemia survival and growth mostly increased in cultures where isolate added as compared to that obtained in Artemia fed WT solely. Especially, the total length of Artemia/screw-cap glass tube was obtained nearly double in the culture where HT1, HT3, HT6 and HT7 added, compared to that obtained in solely WT-fed Artemia. The results of this study indicated that bacteria not only act as a direct food but the presence of these bacteria in the culture water also as water bio-remediator that result in improved survival of Artemia, and bacteria may also improve the availability of yeast nutrients to Artemia that result in the increased length of Artemia.


Artemia, associated bacteria, gnotobiotic, Saccharomyses cerevisiae yeast

Cited as: Toi, H.T., 2017. Effect of associated bacteria on gnotobiotic Artemia performance. Can Tho University Journal of Science. 7: 58-64.

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