Global warming has caused many challenges effects on fermentation industry. Among the promising solutions, isolation of thermololerant lactic acid bacteria from different sources is a good answer to deal with environmental problems. This study aims to isolate and to select strains of lactic acid bacteria which have good thermotolerant and fermentation abilities from agricultural wastes collected from Mekong Delta areas and Binh Duong province, Vietnam. Besides, the morphology, biochemical properties and anti-bacterial activities of lactic acid bacteria were investigated. The thermotolerant and fermentation properties of the strains were also checked to select promising strain produced high lactic acid at high temperatures. As a result, 36 strains of lactic acid bacteria were isolated from samples collected in different areas with corresponding biochemical properties: Gram-positive, negative oxidase and catalase activities, positive acid forming. In thermotolerant test, strains CD2, CD4, ND1, ND2, KD2, KD3, KD5, KC2, and BV1 could grow at high temperature up to 47°C. 29/36 strains of lactic acid bacteria could inhibit the indicated strain of Bacillus subtilis and create clear zones on plates with the diameters ranged from 6.33 to 11 mm. Especially, 5 strains (ND3, ND1, ND4, CT1, BA4) could produce inhibition zone larger than 10 mm. Among 10 strains analyzed for acid fermentation, strain CC2 could ferment medium of sucrose 4% (w/v) and produce the highest total acid concentration at 1.425 g/L after 5 days.