Thong tin chung: Ngay nh?n bai: 04/03/2020
Ngay nh?n bai s?a: 09/06/2020 Ngay duy?t dang: 29/06/2020 Title: The study on matrix metalloproteinase-8 inhibitory activity of Isaria cicadae and Isaria tenuipes extracts isolated in Vietnam T? khoa: Isaria cicadae, Isaria tenuipes, MMP-8, polysaccharide, ?c ch? di can Keywords: Isaria cicadae, Isaria tenuipes, metastases suppression, MMP-8, polysaccharide | ABSTRACT Isaria cicadae (I. cicadae) and Isaria tenuipes (I. tenuipes) are insect parasitic fungi that contain many bioactivites including tumor inhibited activity, anticancer, immune booster. MMP-8 (Matrix Metalloproteinase-8) is one of the collagenases belonging to the endopeptidase enzyme family in mammals, related to extracellular matrix degeneration, angiogenesis, invasion and metastasis. The objective of this study conducted to investigate metastatic inhibitory activity of MMP-8 through restraining collagen resolution using 26 extracts of I. cicadae and I. tenuipes, at concentrations of 20, 200, 2000 ?g/mL. Cordyceps polysaccharide extract (CPS) of I. cicadae fruiting body exhibited the highest of MMP-8 inhibitory cabacity at the concentration of 2000 ?g/mL, with inhibitory ratio was 52.05 ? 0.18%. The results show the MMP-8 inhibitory potential of polysaccharide extract, plant the seed for more in-depth studies on MMP-8, I. cicadae and I. tenuipes in the future. TOM T?T Isaria cicadae (I. cicadae) va Isaria tenuipes (I. tenuipes) la n?m ky sinh con trung ch?a nhi?u h?p ch?t co ho?t tinh sinh h?c bao g?m ho?t tinh ?c ch? kh?i u, khang ung th?, tang c??ng mi?n d?ch. MMP-8 (Matrix Metalloproteinase-8) la m?t trong nh?ng collagenase thu?c endopeptidase ? d?ng v?t co vu, lien quan d?n s? thoai hoa ch?t n?n ngo?i bao, tang sinh m?ch, xam l?n va di can ung th?. M?c tieu c?a nghien c?u nh?m kh?o sat ho?t tinh ?c ch? di can thong qua ?c ch? s? phan gi?i collagen c?a MMP-8 b?ng 26 lo?i cao chi?t c?a hai loai I. cicadae va I. tenuipes, ? cac n?ng d? 20, 200, 2000 ?g/mL. Cao chi?t cordyceps polysaccharide (CPS) I. cicadae qu? th? th? hi?n kh? nang ?c ch? MMP-8 la cao nh?t ? n?ng d? 2000 ?g/mL, v?i t? l? ?c ch? la 52,05 ? 0,18%. K?t qu? cho th?y ti?m nang ?c ch? MMP-8 c?a cao chi?t polysaccharide, lam ti?n d? cho cac nghien c?u chuyen sau h?n v? MMP-8, I. cicadae va I. tenuipes trong t??ng lai. |
