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Kỷ yếu hội nghị quốc tế 2014
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Tạp chí: Diseases in Asian aquaculture, Ho Chi Minh city, 24-28 November 2014
Liên kết:

Outbreak of acute hepatopancreatic necrosis syndrome (AHPNS) appeared in large region and seriously affected to shrimp farming in Vietnam. With intensification in shrimp production, the pressure from AHPNS has greatly increased, some farmers have dealt empirically with AHPNS by using chemicals and antibiotics without laboratory diagnostic support and veterinary supervision. The aim of this study was to determine the sensitivity of 67 Vibrio parahaemolyticus bacterial isolates to 8 common antibiotics used in shrimp farming. These isolates were recovered from shrimp displayed typical pathology of AHPNS such as hepatopancreatic atrophy, empty gut and showed hepatopancreaic changes including dysfunction of hepatopancreatic cells, hemocytic infiltration and bacterial infection. Results of antibiotic susceptibility testing by the disk diffusion method showed that single resistance to tested antibiotics was relatively rare. The majority of isolates tested exhibited multi-resistance to more than one tested antibiotics. The minimum inhibitory concentrations (MICs) for oxytetracyline and florfenicol was also determined for the sensitive isolates using a broth macrodilution method.  MIC value for oxytetracyline of tested isolates was 64 ppm, whereas MIC value for florfenicol was 16-32 ppm. The current study suggests caution in the use of antibiotics for prevention and treatment of AHPNS in shrimp farming and there is an urgent need to apply alternative strategies for prevention and treatment of AHPNS in shrimp farms. 

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