Vibrio spp. including Vibrio harveyi, V. parahaemolyticus, V. pelagicusetc. are the most common and serious pathogen in wild as well as cultured fish and shellfish marine worldwide. Especially V. parahaemolyticus, the specie damaged dramatically the global aquaculture from Asia to North America since 2009. The antibiotic resistance pattern of aetiological agents responsible for V. parahaemolyticus was studied to contribute to control the spread of this bacterial disease in tropical shrimp farming. Bacterial strains isolated from Vietnamese shrimp farms, were assayed for their susceptibility to 16 antibacterial agents currently used in farming practices. A total of 58 V. parahaemolyticusstrains isolated from TraVinh’s shrimp farms were performed using Kirby Bauer and Minimum Inhibitory Concentration (M.I.C.) tests. The bacterial strains showed high sensitivity to doxycycline (84.21%), flumequine sodium (80.70%), norflorxacinHCl (64.91%), and oxytetracycline (49.12%); while that of amoxycylin and cephalexine were completely resistance. Flumequine sodium indicated the lowest M.I.C. value (2μg/mL) of all strains, suggesting its marked antibiotic effect. This study provided useful information about the MIC levels of V. parahaemolyticustowards antibiotic agents in finding cure for AHPND disease in the future.