Aim of the study to pick out the actinomyces and  the fungicides for best effect in controlling vascular wilt caused by Fusarium oxysporum was conducted in laboratory and nethouse conditions. There were three strains actinomyces i.e. 25, 51 and 79 expressed antagonistic ability agains F. oxysporum  from four tested actinomyces 25, 51, 79, 89 in which strain 51 showed  significantly higher fungal inhibition than other strains. When testing two fungicides Benomyl (trade name Binhnomyl) and Difenconazol (trade name Score), both shown ability to inhibit F. oxysporum, Binhnomyl showed higher fungal inhibition zone of F. oxysporum. In nethouse, all three actinomyces i.e. 25, 51, 79 and two fungicides i.e.  Binhnomyl, Score through two pathogen inoculation methods i.e. applying  into soil before sowing or at apply 20 days after sowing with fungal suspension at 2x10⁶ spores/ml expressed effect in reduction vascular wilt  caused by F. oxysporum, strain 25 and Binhnomyl showed  highest disease prevention  early  and last stable  than the other treatments.