A bioassay trial was conducted to determine the efficacy of developed product, Pondguard as an anti-Acute hepatopancreatic Necrosis Disease (AHPND) candidate. The Pondguard (Registration no. D 16060285-HBC) is consisting of natural oils, like, lavender oil, Eucalyptus oil and Pine oil. It maintains the immunity level of shrimp, which helps to protect shrimp from infectious diseases. Two doses of Pondguard i.e. 40 ppm and 80 ppm were selected for the trial. The treatment groups tanks were applied Pondguard whereas no application in control tanks throughout the experiment. The shrimp of both treatment and control were challenged by immersion method. The cumulative mortality reached up to 56.7% in positive control whereas 23.3 % in 80 ppm pondguard group and 13.3% in 40 ppm pondguard group at dpi 8 whereas no mortality recorded in negative control. The Relative Percent Survival of 80 ppm group was 64.7% and of 40 ppm group was 76.5%. The trial results show that the developed Pondguard has significant effect against AHPND-Vibrio parahaemolyticus in a controlled condition.