This study aimed to evaluate the antibacterial and antifungal activities of the ethanolic extract of
Artemisia vulgaris. The results showed that antimicrobial effect against six bacterial strains including
Aeromonas hydrophila, Edwardsiella ictaluri, Enterococcus faecalis, Escherichia coli, Staphylococcus
aureus, Streptococcus agalactiae. However, it had no effectiveness when testing with Pseudomonas
aeruginosa, Aeromonas dhakensis,Vibrio parahaemolyticus. Beside, there were 3 strains fungal
isolated and designated as NS1, NS2 and NS3 from Capsicum frutescens and identified by molecular
biology based on gene sequencing of ITS region. The result showed that the NS1, NS2, NS3 isolated
belong to an Fusarium oxysporum MT453296.1; Lasiodiplodia theobromae JX868613.1,
Colletotrichum scovillei LC577889.1 that with homology were 100 %; 99.24 %; 98.91%, respectively.
The antifungal activity, minimum inhibitory concentration (MIC) of Artemisia vulgaris were examined
for Fusarium oxysporum, Lasiodiplodia theobromae, Colletotrichum scovillei. The result indicated that
MIC of Artemisia vulgaris the most effective against to both fungal hyphae and zoospore of Fusarium
oxysporum and Lasiodiplodia theobromae in 2.5 – 10 mg/mL at 96 hour exposure. These findings
indicated that Artemisia vulgaris is a very potential herb containing lots of natural antibacterial and
antifungal compounds.