The study aimed to evaluate the endemicity and determine the genetic characteristics of canine distemper virus (CDV) in Can Tho City. A study was carried out on 1,120 dogs raised in Can Tho City, and there were 150 dogs showed clinical signs of canine distemper. The result showed 68/150 (45.33%) dogs were positive for CDV, which was determined based on the test kit of Rapid Test Immumo provided by Asan company (Korea). Dogs under 6 months-age had the highest infection rate (68.00%), significantly higher than other age groups (Pstrains belonged to the Asia 1 lineage, commonly found in Vietnam and other Asian countries.