Abstract- A field study was conducted to determine the effect of P-K solubilizing bacteria and nitrogen chemical fertilizer on growth, yield and nitrate in leaf of Brassica juncea L cultivated on acid sulphate soil together with soil fertility as pH soil, N total and organic matter. Twelve P-K solubilizing bacterial strains plus nitrogen chemical fertilizer (0N, 25N and 50N), bacterial liquid were directly watered into plant at 3 stages [6, 12 and 21 days after planting] during vegetable cultivation, chemical fertilizer (100 N – 80 P2O5 – 40 K2O) and control (no-inoculation). The study revealed that twelve P-K solubilizing bacterial strains are PGPR which they have characteristics as nitrogen fixation, phosphate and potassium solubilization, application of more nitrogen chemical concentration, increasing nitrate concentration of leaf and concentration of N total and organic matter in acid sulphate soil. Application of Acinetobacter calcoaceticus NT30 strain plus 25 kgN/ha chemical fertilizer in Brassica juncea cultivation was the best cultural practice because the this model not only supported the highest biomass, low nitrate in leaf of Brassica juncea but also improved soil fertility as high pH soil, N total soil and organic matter. Index Terms- acid sulphate soil, biomass yield, Brassica juncea, nitrogen chemical fertilizer, nitrate in leaf, P-K solubilizing bacteria