Article info.

ABSTRACT

Received 02 Jan 2018
Revised 17 Apr 2018

Accepted 30 Nov 2018

A green and simple method has been successfully developed to synthesize chitosan/Ag nanocomposites using Kumquat extract and River-leaf creeper extract as biological reducing agents. It is indicated to be an eco-friendly and green method, so it is suitable for a feasible synthesis of chitosan/Ag nanocomposites with cost effectiveness. The prepared chitosan/Ag nanocomposites have been characterized by UV-vis, Transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). Result showed those chitosan/Ag nanocomposites have been obtained with average particle size of ~15-25 nm (using kumquat extract) and ~15-41 nm (using river-leaf creeper extract). Moreover, the synthesized chitosan/Ag nanocomposites also showed their efficient antimicrobial activity against Staphylococcus aureus and Escherichia coli. This new combined material has been observed to have significantly higher antimicrobial activity than its components do at their corresponding concentrations. The presence of a small percentage (2.75%, w/w) of metal nanoparticles in the nanocomposite was enough to significantly enhance inactivation of S. aureus and E. coli as compared with unaltered chitosan. Therefore, this eco-friendly method could be competitive and alternative to the existing ones that would be used for synthesis of chitosan/Ag nanocomposites. Thus, it would be highly potential to be used in biomedical applications, opto-electronics and medical devices in the future.

Keywords

Chitosan/silver nanocomposites (CTS/Ag NCPs), Escherichia coli bacteria, green synthesis, Kumquat extract, River-leaf creeper extract, Staphylococcus aureus bacteria

Cited as: Quyen, T.T.B., Hieu, V.N., Khang, P.V.H., Chi, N.T.X., Toan, H.T., Thien,  D.V.H., Thanh, L.H.V. and Tuan, N.T., 2018. Comparative study of chitosan/Ag nanocomposites synthesis and test their antibacterial activity on Staphylococcus aureus and Escherichia coli. Can Tho University Journal of Science. 54(8): 96-104.