This study aimed to evaluate the impact of ergothioniene (ESH) concentrated ex- tract (CE) to lipid anti-oxidation and γ-aminobutyric acid (GABA), γ-oryzanol (GORZ) biosynthesis during germinated brown rice (GBR) processing. To do that 0, 2, 4 and 6% ESH CE was supplemented to soaking stage of brown rice for 6 hr and determined changes about the quality of GBR during processing. The results showed when added 4% of ESH CE to soaking stage, total lipid was stable during four stages of GBR processing as 1.96, 1.87, 1.77, and 1.67 g/100 g corresponding to less increasing peroxide and thiobarbituric acid–reactive substances (TBARs) as 0.53, 0.54, 0.55, and 0.55 meq/kg lipid and 0.21, 0.23, 0.25, and 0.26 µmol TBARs/g while GABA and GORZ increased considerably to 2378.63 mg/kg and 222 mg/kg, respectively. The results indicated that ESH CE helps to increase GABA, GORZ and prevent lipid oxida- tion that contributes to improving the quality of GBR.