Background and Objective: Mango is one of the most important fruits in the Mekong Delta. This study was conducted to investigate the SSR marker which showed a high linkage to peel thickness and sweetness of mango cultivars. Materials and Methods: Ten SSR molecular markers including SSR-18, SSR-20, SSR-23, SSR-28, SSR-41, SSR-51, SSR-52, SSR-59, SSR-62 and SSR-68 on 8 mango cultivars: Hoa Loc, Cat Chu, Aroma, Thanh Ca, Australia, Taiwan, Green Thai and Keo were collected and analyzed. Results: The procedure for extracting mango DNA by CTAB achieves good DNA quality. The PCR analysis on 10 standard markers of SSR showed polymorphisms. The PIC coefficients of the 10 molecular markers ranged from 0.56 (SSR-52) to 0.74 (SSR-23, SSR-41) for mango. The average PIC coefficient of the 10 molecular markers studied is 0.673. Conclusion: The study also showed that 4 standard molecular markers of SSR associated with genes regulating peel thickness and sweetness on 8 mango varieties were: SSR-20, SSR-41 (peel thickness), SSR-51 and SSR-68 (sweetness). Such data contribute to the molecular basis for mango breeding programs.