The present study was performed to assess the possibility of Artemia culture applied bio-floc technology in the Mekong delta of Vietnam. Experiment consisted of two treatments applied bio-floc in the Artemia ponds at salinities of 80 and 100 ppt were compared with two treatments of traditional culture (control) with the same salinity for 6 weeks. Each treatment had 3 replicates. Cassava powder combined with chicken manure were used as a carbon source (C:N>10), which were added to the culture ponds every 3 days to stimulate the development of bio-floc. Result showed that survival and growth of Artemia after 2 weeks of culture were not significant differences among treatments (p>0.05), varying in the range of 65.3 -69.7% and total length of 6.7-7.3 mm, respectively. The female fecundity and Artemia cyst yield in the bio-floc treatments were higher than in the control treatments but there was no statistical difference (P>0.05). Furthermore, the Artemia cyst yields in the 80 ppt treatments were higher than in the 100 ppt treatments. Especially, the concentrations of nitrogen compounds (NO₃, NH₄⁺ and TN) in the bio-floc ponds were lower than in the control ponds. These results revealed that application of bio-floc in Artemia ponds can enhance the Artemia cyst yield as well as improve culture medium that contribute to sustainable Artemia farming in the Mekong delta of Vietnam.