The objectives of this study were analysis of the secondary metabolite products from extract of marine Microbacterium tumbae ND2.7c strain which isolated from sponges at Ha Tien Sea, Kien Giang province, Vietnam. Eleven bioactive compounds were identified in the organic solvent ethyl acetate-hexane. The identification of bioactive chemical compounds based on the peak area, retention time, molecular weight, and molecular formula. GC-MS analysis of Microbacterium tumbae ND2.7c strain revealed the existence of the 11 bioactive compounds had antimicrobial activity as cyclopentasiloxanedecamethyl-, tetrasiloxane 3.5-diethoxy-1.1.1.7.7.7-hexamethyl-3.5- bis(trimethylsiloxy), 1-dodecene, 3-isopropoxy-1.1.1.7.7.7-hexamethyl-3.5.5-tris(trimethylsiloxy) tetrasiloxane, 3,5- di-t-butylphenol, phthalic acid, 3.4-dihydroxymandelic acid 4TMS, 1.6-dioxacyclododecane-7-12-dione, 2-Propyl1-pentanol, 2-(2’.4’.4’.6’.6’.8’.8’-heptamethyltetrasiloxan-2’yloxy)-2.4.4.6.6.8.8.10.10-nonamethyl cyclopentasiloxane and phthalic acid monoethyl ester. These compounds are bioactive secondary metabolites having antimicrobial activity, antifungal, antioxidant, against human pathogenic bacteria.