This study aimed to evaluate the gas, CH4, CO2 and organic matter digestibility affected by 4 different probiotics. It included two in vitro experiments, which were arranged in 2 similar completely randomized designs with 5 treatments and 3 replications. Five treatments of Exp1 were non-probiotic supplementation (NP) the others were 0.25% of Vime-Subtyl, Vime-Bacilac, Biotic and Calphovit supplementation (DM basis) to Para grass (Brachiaria mutica) as a basal substrate. In Exp 2, the same percentage and kinds of probiotic supplementation of Exp 1 were done, however the main substrate were 80 (%DM) Para grass and 20 (%DM) concentrate feed including broken rice and soybean extraction meal and crude protein level in the substrate was fixed of 14.0 % (DM). The results show that in Exp 1, CH4 production at 72h of Vime-Bacilac and Calphovit (04.3 and 93.5 ml/g DOM, respectively) was significantly lower value compared to the others