Fats, oils, and greases are difficult to remove from wastewater treatment systems. This study was conducted to isolate and screen lipid-degrading bacteria from contaminated water samples. The ability of bacteria strains in degrading lipids was tested by growing the bacteria on M1 medium. From three samples of domestic wastewater collected from Ninh Kieu District, Can Tho City, 21 bacterial strains capable of lipolysis were isolated, including 9 strains capable of forming halo rings after 72 h cultured on the M1 medium. The survey resulted on lipase enzyme production showed that the KNT2.2 strain could produce the most effective lipase with an efficiency of 2,670; 2,490 and 6,260 U L-1) after 3, 6 and 9 days of culture, respectively. This study reported that KNT2.2 is a potent strain of bacteria to apply lipase from microorganisms to different industries in the present time.