In this study the effect of long-term storage of boar sperm at various storage temperatures

on sperm motility, survival rate, pH, and bacteria status. Modena-diluted sperm from 6 boars

(Duroc) were stored at 4, 17, and 25°C, respectively. After 24, 72, 120, and 144h of storage, all of

the parameters were determined. The motility, survival rate, and bacteria status of boar sperm

were lowest at 4°C and highest at 25°C. However, the pH of extended sperm was lowest at 25°C

and highest at 4°C. In conclusion, the results from this study show that 17°C is the least harmful

of the three temperatures tested for the long-term liquid storage of boar sperm. The 4°C provided

an advantage in inhibiting the bacteria activity, but it induced cold-shocked injury for boar sperm.

Therefore, we need a further optimum formulator extender for the preservation of boar sperm

below 17oC.