The rice-straw mushroom (Volvariella volvacea) has a distinct flavor, pleasant taste, and rich protein content. It has low production costs and a cropping duration of approximately 45 days—making it an effective means for poverty alleviation for those farmers who grow it. Farmers in Vietnam, the Philippines, and Cambodia grow it. Rice straw is one of the most common substrates used for growing this mushroom. The mushroom can grow well in both outdoor and indoor conditions; however, outdoor cultivation has risks of exposure to rain, wind, and/or high temperatures, all which reduce yield. The yield of indoor mushroom production is higher and more stable, as such, indoor growing is preferred. In addition to cultivation, this chapter also covers straw mushroom characteristics, cultivation principles and techniques, and rice straw substrate preparation.