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Tạp chí quốc tế 2015
Số tạp chí 438(2015) Trang: 1-5
Tạp chí: Aquaculture

Enrofloxacin (ENR) has been widely used to treat bacterial infections in catfish aquaculture. However, little is
known about the elimination of ENR in fish following treatment in aquaculture ponds. The aim of this study
was through on-farm trials to establish the withdrawal period for ENR following treatment of striped catfish
(Pangasianodon hypophthalmus).
Three catfish ponds were treated daily for five consecutive days with medicated pelleted feed containing ENR
(10 mg/kg body weight) following normal farmer procedures. Sampling of catfish muscle/skin for residue analysis
was done one day before the first feeding with medicated feed as well as three times during and five times
after (7, 15, 30, 45, and 200 days) application of medicated feed. ENR and its metabolite ciprofloxacin (CIP)
residues were analyzed by Liquid Chromatography coupled to Mass Spectrometry (LC-MS/MS).
As expected, ENR and CIP residues initially accumulated in mixed muscle/skin and the concentrations were
subsequently reduced during the sampling period. After 45 days (D45), the concentration of total ENR and CIP
was 30.8 ± 4.1 μg/kg. Our results show that a withdrawal time of 45 days is sufficient for striped catfish treated
with ENR, i.e. below the Maximum Residue Limit (MRL) assigned by the Commission of the European Communities
(100 μg/kg) in fish for human consumption but above the action level proposed by the US Food and
Drug Administration (FDA) (5 μg/kg). Further, the 30-days withdraw period declared on the ENR products
sold to catfish farmers is inadequate. The ENR and CIP residues found in skin about 200 days after treatment
warrants follow-up studies, e.g. of possible food safety risks

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