Three consecutive experiments were conducted to assess the potential use of Artemia biomass and gut weed (Enteromorpha sp.) in practical diet for the tiger shrimp (Penaeus monodon) postlarvae. Each experiment consisted of five treatments with three replicates. The diet without containing Artemia and gut weed protein considered as a control treatments used for 3 feeding trials. Experiment 1, fishmeal protein was replaced by increasing levels of Artemia protein in the experimental diet, namely 20, 40, 60 and 80%. Experiment 2, soybean meal protein was substituted by increasing levels of gut weed protein that were 15, 30, 45 and 60%. Experiment 3, combined substitution in corresponding treatments in experiment 1 and 2 that fishmeal protein replaced with Artemia biomass protein and soybean meal protein replaced with gut weed protein. All test diets were formulated to be equivalent in crude protein (40%) and lipid (7%) and lasted for 45 days. Overall, performance of shrimp fed the test diets were better than or equal to those fed the control diet as seen by the better growth rate and feed conversion ratio as well as higher resistance to formalin stress. These results suggested that both Artemia biomass and gut weed can be used as protein sources in practical diets for the tiger shrimp postlarvae, indicating high potential of using locally available of these products as feed ingredients for shrimp feeds in the Mekong delta, Vietnam.