Đăng nhập
 
Tìm kiếm nâng cao
 
Tên bài báo
Tác giả
Năm xuất bản
Tóm tắt
Lĩnh vực
Phân loại
Số tạp chí
 

Bản tin định kỳ
Báo cáo thường niên
Tạp chí khoa học ĐHCT
Tạp chí tiếng anh ĐHCT
Tạp chí trong nước
Tạp chí quốc tế
Kỷ yếu HN trong nước
Kỷ yếu HN quốc tế
Book chapter
Tạp chí quốc tế 2017
Số tạp chí 473(2017) Trang: 468-477
Tạp chí: Aquaculture

The widespread farming of catfish (genus Clarias) hybrids between bighead catfish (C. macrocephalusCm) and the introduced North African Catfish (C. gariepinusCg) in the Mekong Delta, where flooding is common, has raised concerns of introgression of Cg into the native catfish due to backcrossing by escaped hybrids with wild Cm. This study employed novel PCR-RFLP analyses of a mitochondrial (Cytochrome c oxidase subunit I, COI) and two nuclear (Rhodopsin and Tropomyosin) markers and six microsatellite loci to differentiate hybrid individuals from their parental species, and evaluate whether introgression has occurred in wild and cultured Cm populations in the region. Results of marker screening showed that sizes and frequencies of microsatellite alleles differed between the two parental species. Four loci exhibited fixed species-specific differences in allele frequency (monomorphic in Cg and highly polymorphic in Cm). Levels of sequence variation varied at the three genes examined, ranging from 3 to 8% (sequence length 652 bp for COI, 802 bp for Rhodopsin and 925 bp for Tropomyosin). Based on variable sites identified that were not shared between the two species, 3 restriction enzymes (SpeI, XcmI and PflMI) were selected to digest PCR products at species-specific sites within the COI, Rhodopsin and Tropomyosin genes, respectively. Results of PCR-RFLP analyses confirmed that Cm is the maternal lineage of cultured hybrids, while nuclear genes (Rhodopsin and Tropomyosin) and microsatellite loci revealed that hybrids possessed admixed multi-locus genotypes relative to the two parental species. Analyses of six microsatellite loci and PCR-RFLP of two nuclear genes in 473 individuals collected from 11 wild and three cultured populations revealed one F1 hybrid in the wild but no evidence of widespread introgression into native bighead catfish. These results differ from findings in Thailand where intensive catfish culture and deliberate species hybridization is practiced.

 
Các bài báo khác
Số tạp chí 41(2017) Trang: 1591-1607
Tạp chí: Turkish Journal of Mathematics
Số tạp chí 6(2017) Trang: 93-102
Tạp chí: International Journal of Geometry
Số tạp chí 12(2017) Trang: 70-77
Tạp chí: Applications and Applied Mathematics
Số tạp chí 12(2017) Trang: 3651-3664
Tạp chí: FILOMAT
Số tạp chí 5 (4)(2017) Trang: 793-803
Tạp chí: International Journal of Advanced Research
Số tạp chí 5(8)(2017) Trang: 200-207
Tạp chí: International Journal of Advanced Research
Số tạp chí 2 (3)(2017) Trang: 30-48
Tạp chí: European Journal of Foreign Language Teaching
Số tạp chí 02(2017) Trang: 84-90
Tạp chí: International Journal of Advanced Scientific Research and Management


Vietnamese | English






 
 
Vui lòng chờ...