Effects of dietary protein and lipid levels on growth, feed utilization and fillet quality of clown knifefish (Chitala chitala) were implemented to determine the protein and energy requirements of this species at 400 g stage. A 4 x 3 factorial design with four dietary protein levels (25%, 30%, 35% and 40%) and three dietary lipid levels (6, 9, and 12%) corresponded to 3 energies levels 18, 19 and 20 MJ/kg. Clown knifefish (407 ± 5.0 g) was stocked at a density of 10 fish/m². The experiment was arranged in a completely randomized design  in triplicate tanks for 8 weeks. There was a significant interaction effect (P<0.05) between protein and lipid levels on weight gain and specific growth rate with the fish growth being highest in the 35 CP-9% lipid treatment. The survival rate of fish was not affected (P>0.05) by dietary protein and lipid levels and ranged from 93.3 to 100%. The feed efficiency of fish was influenced by dietary protein or lipid content as well as the interaction between these two factors. Protein utilization efficiency increased as protein content increased, and diet contained t 35% protein and 9% lipid,  enhanced chewiness and hardness of fish paste. These results showed that clown knifefish fed 32.8% dietary protein and 19 MJ energy with a P/E ratio of 18.6 had the optimal growth and the best quality of fish meat.