This study was conducted to evaluate the effect of dietary chive supplementation on growth performance, hematological and biochemical blood parameters, and fecal E.coli of growing chickens. One-day-old chickens (Noi Lai breed; n=120) were allocated to a completely randomized design with four replicates of four levels of fresh chives: 0, 1, 1.5 and 2% in the diet (DM basis).There were linear improvements in growth rate and feed conversion of the chickens as the level of chives in the diet increased from 0 to 2% (DM basis) of the diet. There were no effects of chive supplementation on hematological and biochemical indices in blood. E. coli were shown to be susceptible to chive extracts (MIC test); however, supplementation with chives at up to 20g/kg diet DM had no apparent effect on fecal E. coli counts. It is suggested that chives could be considered as a prebiotic for natural growth promotion in chickens.