The experiment was carried out using two factors completely randomized experimental design with four replications per treatment. The first factor included three rice varieties (Akita, Kinu and Hananomai), and second factor included unioculated biofertilizer seed [0,25 g N/pot + 0,15 g P2O5/pot (100 N–60 P2O5 kg/ha)] and inoculated biofertilizer seed [0,125 g N/pot +0,075 g P2O5/pot (50 N–30 P2O5 kg/ha) + nitrogen-fixing bacteria Azospirillum lipoferum and phosphate-disolving bacteria Pseudomonas stutzeri]. The results showed that using the biofertilizer (mixture of two bacterial strains) increased yield, number of grain/panicle, rate of firm rice grain, 1.000 grain weight, HI index, root length.