This study was conducted to determine the prevalence and identification of Salmonella serovars from carcasses, tools and environment at pig slaughterhouse in Can Tho city and examine the sources contaminated Salmonella to carcasses. The Prevalence of Salmonella at the pig slaughterhouse was 13.07%. Out of these, porks were 11.41%, lymphnodes (11.11%), gall bladders (20%), tools and environments (14.76%) and flies (8.41%). The most predominance of 23 Salmonella serovars isolates were S. derby, S. anatum, S. London. There were 42 among 368 carcasses (11.41%) unsatisfying for organism standards. Salmonella was isolated at 17 out of 42 carcasses (40.48%) from carried pigs which harboured serovars such as S. anatum, S. london. There were 25/42 carcasses (59.52%) which showed Salmonella positive from contaminated samples with S. anatum, S. derby, S. london, S. zega. The carried pigs were the sources of Salmonella contamination to carcasses, tools and environment at pig slaughterhouse in Can Tho. The contaminated ways of Salmonella to carcasses at Can Tho pig slaughterhouse were mainly from water, following by tools, flies and environment.