Đăng nhập
 
Tìm kiếm nâng cao
 
Tên bài báo
Tác giả
Năm xuất bản
Tóm tắt
Lĩnh vực
Phân loại
Số tạp chí
 

Bản tin định kỳ
Báo cáo thường niên
Tạp chí khoa học ĐHCT
Tạp chí tiếng anh ĐHCT
Tạp chí trong nước
Tạp chí quốc tế
Kỷ yếu HN trong nước
Kỷ yếu HN quốc tế
Book chapter
Tạp chí quốc tế 2017
Số tạp chí 65(2017) Trang: 235-243
Tạp chí: Fish and Shellfish Immunology

This study aimed to unravel the regulatory roles of choline in activating immune responses and disease resistance of the orange-spotted grouper Epinephelus coioides. Fish were fed a choline-supplemented diet at 1 g kg1 of feed for 30 days. Fish fed a fish meal basal diet without choline-supplement served as controls. At the end of the feeding trial, fish were challenged with Vibrio alginolyticus. Meanwhile, plasma proteomics of fish in each group were also evaluated by two-dimensional gel electrophoresis (2-DE), and differentially expressed proteins were identified by tandem mass spectrophotometry (MS/MS), then a Western blot analysis or real-time polymerase chain reaction was used to confirm differential expressions of immune-enhancing proteins. Results showed that choline significantly increased survival of E. coioides 48 days after being injected with V. alginolyticus. From maps of plasma proteins, a comparative analysis between the control and choline groups revealed that 111 spots matched, with 26 altered expression spots in the choline group. Of these 26 spots, 16 were upregulated and 10 downregulated. After protein identification by reverse-phase nano-high-performance liquid chromatographyelectrospray ionization MS/MS analysis, eight of 26 proteins were found to be immune-related proteins, all of which were upregulated, including complement 3 (C3), alpha-2-macroglobulin-P-like isoform (A2M), fibrinogen beta chain precursor (FBG), and immunoglobulin heavy constant mu (Ighm) proteins. Expression of the A2M protein and A2M enzyme activity in plasma of fish fed choline significantly increased compared to the control group. Additionally, A2M messenger (m)RNA transcripts were also upregulated in the liver and kidneys. Significantly higher C3 expressions at both the mRNA and protein levels were detected in the liver of fish in the choline group. Moreover, FBG gene expressions in the liver and kidneys significantly increased, while Ighm increased in the kidneys and spleen of fish in the choline group. Our results suggest that dietary administration of choline can protect grouper against bacterial infections through activating the complement system, thereby inducing antiprotease activity and natural antibodies that play important roles in the innate immune system of fish.

Các bài báo khác
Số tạp chí 8(2017) Trang: 139-155
Tạp chí: Journal of Modern Methods in Numerical Mathematics
Số tạp chí 103(2017) Trang: 1-12
Tạp chí: Centre for ASEAN Studies
Số tạp chí 14(2017) Trang: 1750021(1-24)
Tạp chí: International Journal of Humanoid Robotics
Số tạp chí 7(2017) Trang: 32-37
Tạp chí: International Journal of Engineering and Innovative Technology (IJEIT)
Số tạp chí 30(2017) Trang: 04017294-1 - 04017294-9
Tạp chí: Journal of Materials in Civil Engineering
Số tạp chí 11(2017) Trang: 24-32
Tạp chí: Australian Journal of Basic and Applied Sciences
Số tạp chí 4(3)(2017) Trang: 14-24
Tác giả: Dương Văn Học
Tạp chí: European Journal of Economics, Law and Politics
Số tạp chí 13(2017) Trang:
Tạp chí: Journal of Communications Software and Systems (JCOMSS)
Số tạp chí 5(2017) Trang: 140–144
Tạp chí: International Journal of Materials Science and Engineering
Số tạp chí 272(2017) Trang:
Tạp chí: IOP Conference Series: Materials Science and Engineering
Số tạp chí 3(2017) Trang: 412-420
Tạp chí: Imperial Journal of Interdisciplinary Research
Số tạp chí 4(2)(2017) Trang: 1-26
Tạp chí: Journal of English Education and Linguistics Studies


Vietnamese | English






 
 
Vui lòng chờ...