The objectives of the study were to determine the effective washing disinfectant (chlorine dioxide and r peracetic acid) in reducing total aerobic counts, E. coli and coliform, and then to test different packaging materials, namely polypropylene (PP), high density polyethylene (HDPE), and low density polyethylene (LDPE), on the quality and shelf-life of Ceylon spinach. The results showed that washing Ceylon spinach with 100 ppm peracetic acid solution for five minutes significantly reduced E. coli and coliform counts (reduction of 1.1 and >2 log CFU g-1, respectively). Ceylon spinach packed in HDPE material might optimally prolong the shelf-life up to 7 days whereas spinach packed in LDPE was recommended if used within 6 days at 10 ± 1oC.