The research has been done in the laboratory of the Plant Protection Department, College of Agriculture and Applied Biology, Can Tho University to screen actinomycetes isolates able to control anthracnose disease caused by Colletotrichum sp. In laboratory condition, the results showed that 3 actinomyces isolates, HG1, HG3 và HG6 could reduce mycelia growth of anthracnose fungus with radiuses of inhibition zones reaches 9.3mm; 10.6mm and 9.8mm and antagonistic efficacy 65.1%; 66.9% và 70.5%, respectively at 9 days after inoculation. In vivo condition, 2 actinomyces isolates HG1 and HG3 which the treatment applied 2 days before and the treatment applied 2 days before and also 2 days after inoculaton showed high ability to control the disease and was not different significantly with Carban 5SC treatment at 14 days after testing.